To investigate the Effect of Varying Temperature on the Rate of Respiration of Germinating Seeds
To investigate the Effect of Varying Temperature on the Rate of Respiration of Germinating Seeds
Description
Germinating seeds actively respire to produce energy for growth. The rate of respiration can be obtained by measuring the uptake of oxygen per unit time.
Apparatus & Materials needed :-
beaker 100ml
beaker 600ml
conical flask 100ml
1 rubber bung fitted with a delivery tube
5g germinating seeds (supplied in petri dish)
1 packet of soda lime wrapped in muslin cloth
coloured water
thermometer
30cm ruler
electronic balance
graph paper
stopwatch }
test tube holder } optional
dropper }
water bath }
soda lime
To prepare germinating seeds need 4days in advance before the experiment.
Day 1 : Soak green beans about 250 grams in 1 litre of water
Day 2 : Place cotton wools or towels onto trays.
Remove green beans from beaker and transfer to trays.
Wet trays just covering the green beans.
Cover the trays with black plastic bags.
Day 3 : Seeds starts to germinate
Day 4 : Germinating seeds for the experiment
Alternatively can buy readily available Alfalfa Sprouts
from supermarkets costly around $3.30 per 125g
from supermarkets costly around $3.30 per 125g
Experimental set up
Procedure
- Weigh 5g of germinating seeds and place them in the conical flask.
- Tie the string to the packet of soda lime.
- Prepare water bath maintained at 30oC.
- Set up the apparatus as shown above.
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